15. Toxicology and Pathology Report from West China University of Medical Sciences: Kang Ershou is safe and non-toxic.

15. West China University of Medical Sciences, Public Health Practical Health Care Trial Report

Sample name: "Kang Er Shou" slimming tea

Sample quantity: 1.5 kg

Submitted items: acute toxicity, weight loss effect

Sample received on April 8, 1996.

Company Name: Chongqing Kangershou Health Food Research Institute

Bulk packaging

Submitter: Deng Shucai

Report date: May 28, 1996

Experimental items and test results:

I. SD rats, weighing 220±10g, animal qualification certificate number: Chuanshi Animal Management Bureau 24101101; Kunming mice, weighing 17±5g, animal qualification certificate number: Chuanshi Animal Management Bureau 67. Half were male and half female.

II. Acute toxicity test: The oral median lethal dose (LD50) of mice and rats was determined using the Horn method. No deaths occurred in mice and rats weighing 10 g/kg, and the LD50 was >10 g/kg. No acute toxicity was observed.

III. Animal Weight Loss Experiment: First, an obese rat model was established. Based on the established animal model, a weight loss experiment was then conducted. Animals were divided into: a normal control group (fed regular feed), an obese control group (fed high-calorie feed), a weight loss group 1 (fed high-calorie feed, 0.5g tea/100g body weight), and a weight loss group 2 (fed high-calorie feed, 1.0g tea/100g body weight). The concentrated tea extract was administered to the test animals via gavage. The weight loss experiment lasted for two weeks.

Observation indicators: weight, body fat weight/body weight ratio (perirenal and retroperitoneal fat), triglycerides, total cholesterol, liver pathology indicators, and whether there are side effects (diarrhea, decreased physical strength).

Results: After the experiment, the average weight gain in the obese control group was significantly higher than that in the normal control group; the weight gain in weight loss group 1 was not significantly different from that in the control group; the weight loss in weight loss group 2 was significantly lower than that in all other groups. The fat-to-body weight ratio was higher in both the obese and weight loss groups 1 than in the normal control group, while there was no significant difference between weight loss group 2 and the normal control group. Cholesterol levels showed no significant differences among the groups. Triglycerides were lower in both weight loss groups than in the obese control group, but still higher than in the normal control group. No significant side effects were observed during the experimental period. Liver pathological examination revealed no abnormalities.

IV. Conclusion: Under experimental conditions, "Kang Er Shou" slimming tea has the effects of weight loss and lowering blood lipids.

Presenter: Li Yun; Person in Charge: Zhang Xu

Report Date: May 28, 1996

XVI. Pathology Laboratory Report from the School of Public Health, West China University of Medical Sciences

--Analytical and Testing Center, School of Public Health, West China University of Medical Sciences

Test items and results: Liver pathological examination of Kangerser experimental rats

Forty-eight rat liver tissue samples were received. Half were male and half female, divided into four experimental groups: a normal control group, an obese group, various dose groups, and a low-dose group. The livers were fixed in 20% formalin, routinely embedded in paraffin, stained with hematoxylin and eosin (HE), and observed under a light microscope. Results showed that the normal control group showed no abnormalities except for mild inflammation in the portal areas. In the obese group, hepatocytes were extremely enlarged with sparse, vacuolated cytoplasm containing large amounts of glycogen granules. Hepatic sinusoids were obstructed, with lesions more prominent in the central and peripheral zones of the hepatic lobules, but no obvious steatosis or necrosis was observed. These lesions were more severe in the male obese group. The amount of glycogen in hepatocytes in the high-dose and low-dose groups showed different gradients of decrease, especially in the female high-dose group. In three out of six animals, the liver tissue morphology and structure were similar to that of normal rats, with a significant reduction in glycogen granules in the hepatocytes, and their volume was similar to that of normal rat hepatocytes. The lobules also tended towards normal.

Speaker: Zhang Yinzhu

Report Date: May 20, 1996